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My file already has a few email addresses in it: is it a problem?

No, it is not an issue. For each row, if there are a “first name”, a “last name” and a “company name” (or a “company domain name”), then Icypeas will search for the email address (even though there is already one in the file). If the row contains only an email address (and no additional information such as “first name”, “last name” and “company name”), then Icypeas will check if this email address is valid or not.